Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the invasive ability of HCT-116 cells. (A) Microscopic observation and photography after crystalline violet staining (100× magnification); (B) the OD values of each group at 570 nm wavelength after dissolution of crystalline violet by sodium acetate; (C-G) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction; OD, optical density.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Staining, Dissolution, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the apoptosis of HCT-116 cells. (A,B) Flow cytometric detection of the effects of different transfection groups on apoptosis of HCT-116 cells; (C-I) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and Western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: British Journal of Cancer

Article Title: DLEC1 and MLH1 promoter methylation are associated with poor prognosis in non-small cell lung carcinoma

doi: 10.1038/sj.bjc.6604452

Figure Lengend Snippet: ( A ) Schematic drawing of the short arm of chromosome 3 and the relative location of the RARβ , MLH1 , DLEC1 , RASSF1A and BLU genes. ( B ) DLEC1 (NM_005106) and GAPDH expression using RT–PCR (two upper panels) and methylation status using MSP (two bottom panels) in lung cancer cell lines and in normal human lung tissue. ( C ) Restoration of DLEC1 expression and concomitant demethylation of the CpG island in H1299 cells using the 5-aza treatment.

Article Snippet: Deleted in lung and oesophageal cancer 1 methylation status was assessed by a fluorescence based real-time detection quantitative methylation-specific PCR (MSP) with primers DLEC-m1, DLEC-m2 ( ) and a TaqMan® probe 5′-6FAM-TAATCAAACTTACGCTCACTTCGTCGCCG-BHQ1-3′ (Biosearch Technology, CA, USA) ( Weisenberger et al , 2006 ).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Methylation